Athurva Gore/LabNotes/2011-4-8

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Revision as of 21:59, 8 April 2011 by >Ajgore
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Bisulfite Biotin-Binding

  • Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA:

File:04072011 Low Input Bisulfite FAILED.jpg

  • Bisulfite-converted libraries did not work.
    • DNA was definitely attached to beads, as normal amplification worked.
    • DNA must have been detached
  • Can we modify the experiment to still allow attachment to beads?
    • According to Invitrogen website [1], bead-detachment can occur when:
      • Heat in formamide or basic solution at 95 for 2 min or 65 for 5 min
      • Boil beads