AlanFung:LabNotes/CTCF/2011-4-25
Jump to navigation
Jump to search
Nextera Methyl-Seq DNA Sample Prep on 100cells (600pg DNA)
Low input transposase-based library preparation (Shendure's Protocol)
Objective
- Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100 cells, ~600pg DNA starting amount) with GM12878 gDNA.
- Adapt Shendure's protocol for low input library preparation
- Improve the yield from the bisulfite conversion step using suggestions made by zymo tech support
- Increase yield from the bisulfite conversion using zymo methylation direct kit (works with 50pg starting amount)
- Test Kapa Hifi polymerase