AlanFung:LabNotes/CTCF/2011-4-25

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Nextera Methyl-Seq DNA Sample Prep on 100cells (600pg DNA)

Low input transposase-based library preparation (Shendure's Protocol)

Objective

  • Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100 cells, ~600pg DNA starting amount) with GM12878 gDNA.
  • Adapt Shendure's protocol for low input library preparation
  • Improve the yield from the bisulfite conversion step using suggestions made by zymo tech support
  • Increase yield from the bisulfite conversion using zymo methylation direct kit (works with 50pg starting amount)
  • Test Kapa Hifi polymerase