Rui:LabNotes/SingleCell/2011-5-20
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Additional 10 cycles of direct lysis samples
- Continue with lab note on 5.19.11
- Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA and 150pg gDNA
QPCR curve
File:Additional 10 cycles 5.20.11.jpg
TBE PAGE
Repeat 2nd strands and PCR with the remaining samples
- Continue with samples on 5.19.11, the remaining 9ul A-tailing product
- 2nd strand with P8
- PCR with 30 cycles