Rui:LabNotes/SingleCell/2011-5-20

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Revision as of 17:14, 23 May 2011 by >RuiLiu (→‎Beads purification)
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Additional 10 cycles of direct lysis samples

  • Continue with lab note on 5.19.11
  • Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA and 150pg gDNA

QPCR curve

File:Additional 10 cycles 5.20.11.jpg

TBE PAGE

File:10 cycles 5.20.11.jpg


Repeat 2nd strands and PCR with the remaining samples

  • Continue with samples on 5.19.11, the remaining 9ul A-tailing product
  • 2nd strand with P8
  • PCR with 30 cycles