AlanFung:LabNotes/CTCF/2011-6-7
Jump to navigation
Jump to search
Protocol
Component | amount per reaction |
distilled water | 35.6 |
10x pfuturbo cx reaction buffer | 5 |
dNTPs(10mM each DNTP) | 1 |
DNA template | 1 |
CHR8 F primer 3.3uM | 3 |
CHR8 R primer 3.3um | 3 |
sybr green 50x | 0.4 |
pfuturbo cx hotstart dna polymerase 2.5U/ul | 1 |
total reaction volume | 50 |