Rui:Collaborations
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Dr. Yi Zhang lab
Samples
- Sperms: ~50cell/tube; 15 tubes
- ES cells: ~50cell/tube; 15 tubes
- E9.5 bag: ~641 cells in 1 tube; ~316 cells in 1 tube.
- E11.5 bag: ~21550 cells in 1 tube.
- E13.5 bag: ~30078 cells in 1 tube (male PGCs); ~57059 cells in 1 tube (female PGCs)
Quick note
MmeI primer digestion [1]
50 cells RNAseq | procedure | samples | start | end | note |
~25 cells | RNA or lysate | Sperm vs ES cells | 5.19.11 | 5.20.11 | |
process, clone | 5.21.11 | 5.24.11 | 5.25.11 seq result | ||
Nextera library | 5.23.11 | 5.23.11 | spread out (clone?) | ||
Solexa library | 5.23.11 | 5.23.11 | amp. Not good | ||
5.26.11 | 5.26.11 | again. Not good | |||
6.1.11 | 6.2.11 | different buffs | |||
6.2.11 | 6.3.11 | another 2ug repeat | |||
6.4.11 | Kapa for NGS |
2011
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