Rui:Collaborations

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Revision as of 19:59, 24 June 2011 by >RuiLiu (→‎Quick note)
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Dr. Yi Zhang lab

Samples

  1. Sperms: ~50cell/tube; 15 tubes
  2. ES cells: ~50cell/tube; 15 tubes
  3. E9.5 bag: ~641 cells in 1 tube; ~316 cells in 1 tube.
  4. E11.5 bag: ~21550 cells in 1 tube.
  5. E13.5 bag: ~30078 cells in 1 tube (male PGCs); ~57059 cells in 1 tube (female PGCs)

Quick note

MmeI primer digestion [1]

50 cells RNAseq procedure samples start end note
~25 cells RNA or lysate Sperm vs ES cells 5.19.11 5.20.11
process, clone 5.21.11 5.24.11 5.25.11 seq result
Nextera library 5.23.11 5.23.11 spread out (clone?)
Solexa library 5.23.11 5.23.11 amp. Not good
5.26.11 5.26.11 again. Not good
6.1.11 6.2.11 different buffs
6.2.11 6.3.11 another 2ug repeat
6.4.11 Kapa for NGS

2011

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