Rui:Collaborations
Jump to navigation
Jump to search
Samples
Dr. Yi Zhang lab
- Sperms: ~50cell/tube; 15 tubes
- ES cells: ~50cell/tube; 15 tubes
- E9.5 bag: ~641 cells in 1 tube; ~316 cells in 1 tube.
- E11.5 bag: ~21550 cells in 1 tube.
- E13.5 bag: ~30078 cells in 1 tube (male PGCs); ~57059 cells in 1 tube (female PGCs)
Dr. Xu Yang lab
- GFP(+): Pancreatic lineage differentiated from hESC, NGN3+ (~50000 cells, purified with Qiagen RNeasy), 20.8ug/ml, use 1ul
- GFP(-): Pancreatic lineage differentiated from hESC, NGN3- (~50000 cells, purified with Qiagen RNeasy), 12.7ug/ml, use 1ul
- 7/7/11: Origin (?) Method (cell sorting --> spin top speed for at least 5min, done by Dae-Hwan)
Negative control (No fluorescence)--- 2 tubes RFP positive ------ 1 tube GFP/RFP double positive ------ 1 tube
Quick note
MmeI primer digestion [1]
50 cells RNAseq | procedure | samples | start | end | note |
~25 cells | RNA or lysate | Sperm vs ES cells | 5.19.11 | 5.20.11 | |
process, clone | 5.21.11 | 5.24.11 | 5.25.11 seq result | ||
Nextera library | 5.23.11 | 5.23.11 | spread out (clone?) | ||
Solexa library | 5.23.11 | 5.23.11 | amp. Not good | ||
5.26.11 | 5.26.11 | again. Not good | |||
6.1.11 | 6.2.11 | different buffs | |||
6.2.11 | 6.3.11 | another 2ug repeat | |||
6.4.11 | Kapa for NGS |
2011
<calendar> name=Rui:LabNotes/Collaborations format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>