Rui:Collaborations

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Revision as of 19:42, 8 July 2011 by >RuiLiu
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Samples

Dr. Yi Zhang lab

  1. Sperms: ~50cell/tube; 15 tubes
  2. ES cells: ~50cell/tube; 15 tubes
  3. E9.5 bag: ~641 cells in 1 tube; ~316 cells in 1 tube.
  4. E11.5 bag: ~21550 cells in 1 tube.
  5. E13.5 bag: ~30078 cells in 1 tube (male PGCs); ~57059 cells in 1 tube (female PGCs)

Dr. Xu Yang lab

  1. GFP(+): Pancreatic lineage differentiated from hESC, NGN3+ (~50000 cells, purified with Qiagen RNeasy), 20.8ug/ml, use 1ul
  2. GFP(-): Pancreatic lineage differentiated from hESC, NGN3- (~50000 cells, purified with Qiagen RNeasy), 12.7ug/ml, use 1ul
  3. 7/7/11: Origin (?) Method (cell sorting --> spin top speed for at least 5min, done by Dae-Hwan)
Negative control (No fluorescence)--- 2 tubes
RFP positive ------ 1 tube
GFP/RFP double positive ------ 1 tube  

Quick note

MmeI primer digestion [1]

50 cells RNAseq procedure samples start end note
~25 cells RNA or lysate Sperm vs ES cells 5.19.11 5.20.11
process, clone 5.21.11 5.24.11 5.25.11 seq result
Nextera library 5.23.11 5.23.11 spread out (clone?)
Solexa library 5.23.11 5.23.11 amp. Not good
5.26.11 5.26.11 again. Not good
6.1.11 6.2.11 different buffs
6.2.11 6.3.11 another 2ug repeat
6.4.11 Kapa for NGS

2011

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