Brandon:Protocols/Count Cells

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Revision as of 00:06, 23 August 2011 by >Bsos (Created page with '*machine is on 3rd floor *if machine has not been used, need to prime it first. *add in 100 uL of cells into the cup, and 10 mL of "balanced electrolyte solution" **it is one pu…')
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  • machine is on 3rd floor
  • if machine has not been used, need to prime it first.
  • add in 100 uL of cells into the cup, and 10 mL of "balanced electrolyte solution"
    • it is one pullup/pushdown on the handle
  • dish setup
    • set cell sizes to count
    • start run, machine counts 3 times and averages the results (outputs result in cell/mL)