AlanFung:LabNotes/CTCF/2011-8-26

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Revision as of 23:26, 26 August 2011 by >Alan6017518 (Created page with '===Make New BSA-Water solution=== *Stock solution is at 10mg/ml, need to dilute to 0.5mg/ml *Take 10ul stock BSA dilute with 190ul nuclease free water ===Bisulfite Conversion - I…')
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Make New BSA-Water solution

  • Stock solution is at 10mg/ml, need to dilute to 0.5mg/ml
  • Take 10ul stock BSA dilute with 190ul nuclease free water

Bisulfite Conversion - Imprint Kit

  • Prepare DNA Modification Solution (CT Conversion Reagent):
  • Add 21 uL 0.5 mg/mL BSA and 1 uL Imprint Balance solution to each sample; incubate at 37 C for 10 minutes
  • Add 125 uL DNA Modification solution to each tube, incubate at 65 C for 90 minutes
  • Perform Column Purification of each sample:
    • Elute into 11uL Elution Buffer
  • Denature all samples at 95C for 30sec and quickly transfer to cooler rack