AlanFung:LabNotes/CTCF/2011-8-26

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Revision as of 06:19, 27 August 2011 by >Alan6017518 (→‎Bisulfite Conversion - Imprint Kit)
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Make New BSA-Water solution

  • Stock solution is at 10mg/ml, need to dilute to 0.5mg/ml
  • Take 10ul stock BSA dilute with 190ul nuclease free water

Bisulfite Conversion - Imprint Kit

  • Prepare DNA Modification Solution (CT Conversion Reagent):
  • Add 21 uL 0.5 mg/mL BSA and
  • Add1 uL Imprint Balance solution to each sample;
  • incubate at 37 C for 10 minutes
  • Add 125 uL DNA Modification solution to each tube, incubate at 65 C for 90 minutes
  • Perform Column Purification of each sample:
    • Elute into 11uL Elution Buffer

Continue on 8/27

  • Denature all samples at 95C for 30sec and quickly transfer to cooler rack