AlanFung:LabNotes/CTCF/2011-10-4

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Revision as of 23:13, 4 October 2011 by >Alan6017518 (Created page with '==Notes== *From previous experiment, it is verified that cells can be digested using NaOH. I will repeat experiment with the following improvements. **NaOH cell digestion, avoid …')
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Notes

  • From previous experiment, it is verified that cells can be digested using NaOH. I will repeat experiment with the following improvements.
    • NaOH cell digestion, avoid using NP40 and protease, save time and effort.
    • EtOH precipitation after MDA, prevent size elimination with column purification and avoid spending time to speedvac to cut down the sample volume for tagmentation