AlanFung:LabNotes/CTCF/2011-10-4
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Notes
- From previous experiment, it is verified that cells can be digested using NaOH. I will repeat experiment with the following improvements.
- NaOH cell digestion, avoid using NP40 and protease, save time and effort.
- EtOH precipitation after MDA, prevent size elimination with column purification and avoid spending time to speedvac to cut down the sample volume for tagmentation