Alice:LabNotes/2008-7-1

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Experiment 1: Cosmic probe preparation using iTaq buffer

Real time PCR reaction system:
                                                                x12
H2O                                                75.1ul      901.2 ul
10X iTaq buffer                                      10ul        120 ul
MgCl2(25mM)                                           6ul         72 ul
50x SYBG I                                          0.4ul        4.8 ul
dNTP(2.5mM)                                           8ul         96 ul       
primer mix (100uM)                                  0.2ul        2.4 ul
template(cosmic 3760)                               0.1ul        1.2 ul
Platium Taq polymerase                              0.2ul        2.4 ul
Total                                               100ul       1200 ul
PCR program: 94c 2min -> 94c 30sec -> 60C 2min -> 72C 1min-> Plate read -> go to step 2 x 21 cycles -> 72C 5min -> 15C hold.