Alice:LabNotes/2008-7-1
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Experiment 1: Cosmic probe preparation using iTaq buffer
Real time PCR reaction system: x12 H2O 75.1ul 901.2 ul 10X iTaq buffer 10ul 120 ul MgCl2(25mM) 6ul 72 ul 50x SYBG I 0.4ul 4.8 ul dNTP(2.5mM) 8ul 96 ul primer mix (100uM) 0.2ul 2.4 ul template(cosmic 3760) 0.1ul 1.2 ul Platium Taq polymerase 0.2ul 2.4 ul Total 100ul 1200 ul PCR program: 94c 2min -> 94c 30sec -> 60C 2min -> 72C 1min-> Plate read -> go to step 2 x 21 cycles -> 72C 5min -> 15C hold.