Rui:LabNotes/Haplotyping/2011-11-3
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Test condition for haplotyping
- Start with ~1pg cell lysate aliquot
- Perform limited time MDA
- Tagmentation and PCR
- Need to achieve ~1000X increase. Based on 50cell experiments, it seems 1k-2k increase after 2hrs when QPCR shoots up around 12ct and reach the saturation at 20ct.
- Key point is when to stop MDA based on QPCR curve
Samples
Procedures
Results
Ctrls File:11.3.11 Ctrls.jpg
stop at 17ct, #2 up File:11.3.11 17ct.jpg stop at 22ct, #3 up File:11.3.11 22ct.jpg
stop at 27ct, #3,4 start to up File:11.3.11 27ct.jpg stop at 30ct, all start up File:11.3.11 30ct.jpg