Rui:Cell aliquot

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Revision as of 20:53, 4 November 2011 by >Sam Chiang (Created page with '===Cell aliquot=== * 11.3.11 GM112878-P28 (~600k cells/ml) GM20431-P20 (~550k cells/ml) # 1ml cells, 750g, 3min # wash pellet with PBS # resuspend in 1ml PBS # 10ul cells + 9…')
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Cell aliquot

  • 11.3.11

GM112878-P28 (~600k cells/ml)

GM20431-P20 (~550k cells/ml)

  1. 1ml cells, 750g, 3min
  2. wash pellet with PBS
  3. resuspend in 1ml PBS
  4. 10ul cells + 90ul PBS -> ~50-60 cells/ul
  5. 200c strips (2) 4ul/well
  6. 100c strips (2) 2ul/well
  7. 50c strips (2) 1ul/well
  8. 10ul cells + 40ul PBS -> ~10 cells/ul
  9. 10c strips (4) 1ul/well
  • all strips put in tip-box at -80C