Rui:SingelCell RNAseq

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Revision as of 19:14, 14 November 2012 by >Jdeng (→‎PCR note)
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Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes

IVT note

IVT_based description start result
1st try 3 sets 2.8.11 2.9.11
Following purification? 2.10.11
primer check 2.15.11
clone 2.17.11 2.24.11
clone troubleshoot 2.28.11
2nd round IVT 3.2.11 3.3.11
trouble shooting 3.3.11 3.4.11
Atailed_ds cDNA IVT 3.2.11 3.3.11
2nd try repeat kit 3.8.11 3.9.11
3.10.11 3.11.11
Atailed_ds cDNA IVT 4.5.11 4.6.11


HRCA note

HRCA description start result
1st try P1 2.3.11 2.4.11

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>


2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>