Rui:SingelCell RNAseq

From ZhangLabWiki
Revision as of 04:21, 24 April 2013 by >RuiLiu (→‎Protocols)
Jump to navigation Jump to search

SCAP project

  • Main link [1]
  • Libraries [2]
  • Data: genome-miner.ucsd.edu:/media/Ext4T/KZ_Ext4T/RNAseq/
  • Haiyang's link [3]

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes

2013

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2013/10/1 view=oneyear </calendar>

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>