AlanFung:LabNotes/Methylome/2012-1-17
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Contents
1
GM12878 Library
2
Sample Preparation
3
End repair & A-tailing
4
ligation to methylated Y adaptor
5
Bisulfite Conversion
6
PCR
7
Size selection
GM12878 Library
Sample Preparation
Covaris shearing of 5ug gDNA to ~400bp
Contacted Yang at Kelly Frazer's lab to help me with it
End repair & A-tailing
ligation to methylated Y adaptor
Bisulfite Conversion
PCR
Size selection
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