AlanFung:LabNotes/Methylome/2012-1-17
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GM12878 Library
Sample Preparation
- Get GM12878 samples from Noi
- Measure concentration with qubit
- Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
- Covaris shearing of 5ug gDNA to ~400bp
- Contacted Yang at Kelly Frazer's lab to help me with it
End repair & A-tailing
Content | Volume |
Fragmented DNA | variable |
End Reapir buffer | 10 |
End Repair Enzyme Mix | 5 |
ddH2O | Variable |
Volume | 100 |