AlanFung:LabNotes/Methylome/2012-1-17

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Revision as of 22:57, 17 January 2012 by >Alan6017518 (→‎End repair & A-tailing)
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GM12878 Library

Sample Preparation

  • Get GM12878 samples from Noi
  • Measure concentration with qubit
  • Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
  • Covaris shearing of 5ug gDNA to ~400bp
  • Contacted Yang at Kelly Frazer's lab to help me with it

End repair & A-tailing

Content Volume
Fragmented DNA variable
End Reapir buffer 10
End Repair Enzyme Mix 5
ddH2O Variable
Volume 100

ligation to methylated Y adaptor

Bisulfite Conversion

PCR

Size selection