Rui:LabNotes/Collaborations/2012-2-23

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Revision as of 17:37, 24 February 2012 by >RuiLiu (Created page with '==Beta cell samples (repeat)== ===Samples=== * New patch of cells Wei sent to us ===Procedure=== # RNA isolation from cells using ZYMO microPrep kit # EtOH precipitation (visib…')
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Beta cell samples (repeat)[edit]

Samples[edit]

  • New patch of cells Wei sent to us

Procedure[edit]

  1. RNA isolation from cells using ZYMO microPrep kit
  2. EtOH precipitation (visible pellet seen with Glycoblue)
  3. cDNA synthesis using SMARTer kit (brand new kits)

Results[edit]

Nothing including PC after 22 cycles

File:2.23.12 RNA.jpg