Rui:LabNotes/Collaborations/2012-2-23
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Beta cell samples (repeat)[edit]
Samples[edit]
- New patch of cells Wei sent to us
Procedure[edit]
- RNA isolation from cells using ZYMO microPrep kit
- EtOH precipitation (visible pellet seen with Glycoblue)
- cDNA synthesis using SMARTer kit (brand new kits)
Results[edit]
Nothing including PC after 22 cycles