Brandon:Protocols/primers prep

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Revision as of 22:33, 24 February 2012 by >Bsos (Created page with '==Primers preparation== *for each umol (um) of primer add 10 uL nuclease free H2O. ie 35.5 um, add 355 uL nuclease free water. now is at stock concentration of 100 uM. *next di…')
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Primers preparation

  • for each umol (um) of primer add 10 uL nuclease free H2O. ie 35.5 um, add 355 uL nuclease free water. now is at stock concentration of 100 uM.
  • next dilute to usable [] of 10 uM with 10X dilution.
  • combine F/R primers in one tube for usable primer.