Brandon:Protocols/Lysis buffers

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Revision as of 00:32, 30 March 2012 by >Bsos (Created page with '==lysis buffers== ===lysis buffer 1=== *Crawford et al. PNAS 2003 *lysis buffer composition: 10X lysis buffer 100mM Tris.Hcl pH 7.5 100mM NaCl 30mM MgCl2 1% NP40 *make 1M…')
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lysis buffers

lysis buffer 1

  • Crawford et al. PNAS 2003
  • lysis buffer composition:
10X lysis buffer 
100mM Tris.Hcl pH 7.5
100mM NaCl
30mM MgCl2
1% NP40
  • make 1M solution of NaCl and MgCl2
1M NaCl2, 2.922 grams in 50 mL nuclease free water
1M MgCl2, 4.761 grams in 50 mL nuclease free water (add mgcl2 to water, very exothermic!!)
  • To make 10 mL of lysis buffer
500   uL of 2M Tris.Hcl PH7.5
1000  uL of 1M NaCl
333   uL of 1M MgCl2
10    uL of 1% NP40
8.157 mL Nuclease free water