Jie:LabNotes/CpgSeq/2008-8-1

From ZhangLabWiki
Revision as of 22:50, 31 July 2008 by KunZhang (talk | contribs) (New page: ==amplification of spacer== == PCR amplification == reaction system x96 H2O 80...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

amplification of spacer

PCR amplification

 reaction system                                                  x96        
 H2O                                                80.7ul     7747.2ul  
 10x buffer                                           10ul      960ul
 MgCl2(25mM)                                           6ul      576ul    
 dNTP(10mM each, NEB)                                  2ul      192ul
 spacer_100bp_F(100uM)                               0.2ul      192ul
 spacer_100bp_R(100uM)                               0.2ul      19.2ul 
 50x SYBG I                                          0.4ul      38.4ul  
 template(88.3ng/ul, 5/22)                           0.1ul      19.2ul
 Jumpstart Taq                                       0.6ul      57.6ul
 Total                                               100ul
 94C 3min -> 13cycles of (94C 45sec -> 55C 45sec -> 72C 45sec) -> 72C 3min -> 4C