Jie:LabNotes/CpgSeq/2008-8-1
Jump to navigation
Jump to search
amplification of spacer
PCR amplification
reaction system x96 H2O 80.7ul 7747.2ul 10x buffer 10ul 960ul MgCl2(25mM) 6ul 576ul dNTP(10mM each, NEB) 2ul 192ul spacer_100bp_F(100uM) 0.2ul 192ul spacer_100bp_R(100uM) 0.2ul 19.2ul 50x SYBG I 0.4ul 38.4ul template(88.3ng/ul, 5/22) 0.1ul 19.2ul Jumpstart Taq 0.6ul 57.6ul Total 100ul
94C 3min -> 13cycles of (94C 45sec -> 55C 45sec -> 72C 45sec) -> 72C 3min -> 4C