Rui:LabNotes/DNA Collaborations/2012-5-10
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Continue with D2 and D3
- EtOH precipitation o/n
- Spin down with 3700 rpm, really difficult handling with pellet - need to be careful. Resupend in 5ul, 1ul for AluPCR - failed to amplify (due to primer pre-mix?? why??)
- 2ul DNA for Tn tagmentation in 5ul total
- Protease treatment for 30min
- PCR with adaptor 1 and barcoded adaptor 2
- Beads purification for 48 samples
- Gel quantification of 48 samples, 24 for one library
- Mixed library for size selection