Noi/NOTES/2012-5-16

From ZhangLabWiki
Revision as of 20:27, 16 May 2012 by >Noi
Jump to navigation Jump to search

Probe production for the HiResChrPaint project

 Probe IDs    Conc. (ng/ul) Yields in 120ul (ug) Left over after trial (ug)
#1-V4	         83.70	         10.04	                 5.00
#2-V4	        125.10	         15.01	                12.00
#3-V4	        110.10	         13.21	                13.20
#4-V4	        133.80	         16.06	                 8.00
#1-V6	        122.50	         14.70	                14.70
#2-V6	        106.20           12.74	                12.74
#3-V6	        132.20	         15.86	                15.86
#4-V6	        101.70	         12.20	                12.20
DpnII digestion reaction set up
Probe IDs Conc. (ng/ul) Volume (ul) H2O (ul) 10X DpnII Buffer DpnII Total volume (ul) # of tube
#1-V4 83.70 59.74 22.26 10 8 100.00 1
#2-V4 125.10 47.96 34.04 10 8 100.00 2
#3-V4 110.10 59.95 22.05 10 8 100.00 2
#4-V4 133.80 59.79 22.21 10 8 100.00 1
#1-V6 122.50 60.00 22.00 10 8 100.00 2
#2-V6 106.20 59.98 22.02 10 8 100.00 2
#3-V6 132.20 59.98 22.02 10 8 100.00 2
#4-V6 101.70 59.98 22.02 10 8 100.00 2
37C 30min -> 65C 20min -> Qiaquick column purification (also combine the samples used for DpnII digestion trial.
Measure concentration by Nanodrop and calculate yield