Brandon:Protocols/taq amplification2

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Revision as of 19:13, 20 May 2012 by >Bsos (→‎Standard PCR cycling methods for different enzymes/procedures)
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Standard PCR cycling methods for different enzymes/procedures[edit]

  • KAPA SYBR
12.5 uL KAPA SYBR
2    uL primers, 1 uL F, 1 uL R
5.5  uL H2O
5    uL DNA template

KAPA SYBR cycles:
98C 3min, (98C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever


  • taq amplification
    • also used for colony PCR, pick colony and put directly into the reaction

used taq2x for amplification, X35 cycles

12.5 uL taq2X
2    uL primers, 1 uL F, 1 uL R
5.5  uL H2O
5    uL DNA template

taq2x cycles:
95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever


  • KAPA2G Robust