Brandon:Protocols/taq amplification2
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Standard PCR cycling methods for different enzymes/procedures[edit]
- KAPA SYBR
12.5 uL KAPA SYBR 2 uL primers, 1 uL F, 1 uL R 5.5 uL H2O 5 uL DNA template KAPA SYBR cycles: 98C 3min, (98C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever
- taq amplification
- also used for colony PCR, pick colony and put directly into the reaction
used taq2x for amplification, X35 cycles
12.5 uL taq2X 2 uL primers, 1 uL F, 1 uL R 5.5 uL H2O 5 uL DNA template taq2x cycles: 95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever
- KAPA2G Robust