Revision as of 16:26, 20 May 2012 by >Noi
DMR 330k BSPP capture of samples from Sergio
Sample list
on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-17
No.
|
Sample ID
|
Lab ID
|
Conc. (ng/ul)
|
Volume (ul)
|
Yields (ug)
|
1 |
HUES2 p41 |
Ap15-1 |
110.0 |
250 |
27.5
|
2 |
ASThiPS4F1 p12 |
Ap15-2 |
139.1 |
250 |
34.8
|
3 |
ASThiPS4F2 p12 |
Ap15-3 |
145.1 |
250 |
36.3
|
4 |
HUES6 p39 Control TeSR |
Ap15-4 |
295.5 |
250 |
73.9
|
5 |
HUES6 p39 ACTIVIN |
Ap15-5 |
128.1 |
250 |
32.0
|
6 |
HUES6 p39 BMP-4 |
Ap15-6 |
153.9 |
250 |
38.5
|
7 |
HUES9 p39 Control TeSR |
Ap15-7 |
183.5 |
250 |
45.9
|
8 |
HUES9 p39 ACTIVIN |
Ap15-8 |
149.3 |
250 |
37.3
|
9 |
HUES9 p39 BMP-4 |
Ap15-9 |
111.2 |
250 |
27.8
|
10 |
HUES3 p36 Control TeSR |
Ap15-10 |
153.5 |
250 |
38.4
|
11 |
HUES3 p36 ACTIVIN |
Ap15-11 |
87.5 |
250 |
21.9
|
12 |
HUES3 p36 BMP-4 |
Ap15-12 |
109.7 |
250 |
27.4
|
13 |
H1 p54 Control TeSR |
Ap15-13 |
191.3 |
250 |
47.8
|
14 |
H1 p54 ACTIVIN |
Ap15-14 |
108.7 |
250 |
27.2
|
15 |
ASThiPS4F5 p16 Control TeSR |
Ap15-15 |
134.2 |
250 |
33.6
|
*16 |
ASThiPS4F5 p16 ACTIVIN |
Ap15-16 |
50.2 |
500 |
25.1
|
17 |
ASThiPS4F4 p36 Control TeSR |
Ap15-17 |
183.0 |
250 |
45.8
|
18 |
ASThiPS4F4 p36 ACTIVIN |
Ap15-18 |
82.6 |
250 |
20.7
|
19 |
ASThiPS4F4 p36 BMP-4 |
Ap15-19 |
263.4 |
250 |
65.9
|
20 |
H1 p48 |
Ma15-2 |
215.2 |
200 |
43.0
|
21 |
H9 p47 |
Ma15-3 |
252.0 |
200 |
50.4
|
22 |
HUES3 p33 |
Ma15-4 |
226.1 |
200 |
45.2
|
23 |
HUES6 p26 |
Ma15-5 |
238.7 |
200 |
47.7
|
24 |
HUES8 p35 |
Ma15-6 |
83.4 |
200 |
16.7
|
25 |
HUES9 p36 |
Ma15-7 |
236.8 |
200 |
47.4
|
26 |
ASThiPS4F3 p24 (derived from Astrocytes) |
Ma15-8 |
175.9 |
200 |
35.2
|
27 |
ASThiPS4F4 p16 (derived from Astrocytes) |
Ma15-9 |
164.3 |
200 |
32.9
|
28 |
FiPS4F2 p32 (derived from IMR90 fibroblasts) |
Ma15-10 |
205.9 |
200 |
41.2
|
29 |
FiPS4F5 p29 (derived from IMR90 fibroblasts) |
Ma15-11 |
97.6 |
200 |
19.5
|
30 |
KiPS4FA p54 (derived from keratinocytes) |
Ma15-12 |
129.5 |
200 |
25.9
|
31 |
Huv-iPS4F6 p23 (derived from HUVECs) |
Ma15-13 |
263.1 |
200 |
52.6
|
32 |
IMR90 p6 |
Ma15-14 |
76.1 |
200 |
15.2
|
33 |
Keratinocytes p3 |
Ma15-15 |
138.7 |
200 |
27.7
|
34 |
H1 p47 Control |
Ma15-16 |
216.4 |
200 |
43.3
|
35 |
H1 p47 BMP-4 |
Ma15-17 |
137.6 |
200 |
27.5
|
36 |
ASThiPS4F5 p12 Control |
Ma15-18 |
224.6 |
200 |
44.9
|
37 |
ASThiPS4F5 p12 BMP-4 |
Ma15-19 |
111.7 |
200 |
22.3
|
38 |
Huv-iPS4F6 p23 Control |
Ma15-20 |
289.9 |
200 |
58.0
|
39 |
Huv-iPS4F6 p23 BMP-4 |
Ma15-21 |
172.9 |
200 |
34.6
|
40 |
Huv-iPS4F6 p23 ACTIVIN |
Ma15-1 |
86.7 |
250 |
21.7
|
Old gDNA of samples have been captured in the previous experiments
- Note:
- Some of gDNA concentrations were re-measured and note that some of them have conc. lower than 50ng/ul, and some of them don't have enough amount for bisulfite conversion
- Samples KERAT MMTA and KiPS4F #8 have high conc., but very little volume. I added ~17ul H2O then re-measured conc. again
- ***I may not do bis-cvt on the two samples, SR-21 and SR22 since the amount of gDNA was too little
No
|
Sample IDs
|
Lab IDs
|
Conc. (ng/ul)
|
1 |
H9 p43 mTeSR |
SR-1 |
62.8
|
2 |
H9 p43 ACTIVIN |
SR-2 |
90.8
|
3 |
H9 p43 BMP4 |
SR-3 |
93.7
|
4 |
HUV0iPS4F1 p19 |
SR-4 |
98.0
|
5 |
HUV-hiPS4F1 p20 ACTIVIN |
SR-5 |
84.8
|
6 |
HUV-hiPS4F1 p20 BMP4 |
SR-6 |
78.6
|
7 |
HUV-iPS4F3 F19 |
SR-7 |
96.9
|
8 |
HUV-hiPS4F3 p20 ACTIVIN |
SR-8 |
91.9
|
9 |
HUV-hiPS4F3 p20 BMP4 |
SR-9 |
80.6
|
10 |
aMSCs p7 |
SR-10 |
184.8
|
11 |
MSCiPS #8 p14 (~4 ul) |
SR-11 |
105.4
|
12 |
MSCiPS #4 p14 |
SR-12 |
181.4
|
13 |
hNiPS2F p23 |
SR-13 |
270.0
|
14 |
hKiPS3F-7 p24 (~15ul) |
SR-14 |
33.0
|
15 |
hNSC |
SR-15 |
67.0
|
16 |
hFiPS4F-7 p9 |
SR-16 |
145.6
|
17 |
HUVEC p2 |
SR-17 |
245.3
|
18 |
hiPS AST4F-4 p9 |
SR-18 |
89.9
|
19 |
KERAT MMTA |
SR-19 |
159.5
|
20 |
KiPS4F #8 |
SR-20 |
195.7
|
21 |
***olf MSC 19.1ng/ul volume only 3-5ul |
SR-21 |
19.1
|
22 |
***KiPS 14ng/ul volume only 3-5ul |
SR-22 |
14.0
|
8 samples of bis-cvt gDNA from Dinh
- The list will be filled later and need to re-measure of the conc. with ssDNA Qubit assay