Noi/NOTES/2012-5-20

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Revision as of 16:26, 20 May 2012 by >Noi (Created page with "* http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''' = DMR 330k BSPP capture of samples from Sergio = == Sample list == == New gDNA...")
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DMR 330k BSPP capture of samples from Sergio

Sample list

New gDNA extracted from the cell pellet

on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-17
No. Sample ID Lab ID Conc. (ng/ul) Volume (ul) Yields (ug)
1 HUES2 p41 Ap15-1 110.0 250 27.5
2 ASThiPS4F1 p12 Ap15-2 139.1 250 34.8
3 ASThiPS4F2 p12 Ap15-3 145.1 250 36.3
4 HUES6 p39 Control TeSR Ap15-4 295.5 250 73.9
5 HUES6 p39 ACTIVIN Ap15-5 128.1 250 32.0
6 HUES6 p39 BMP-4 Ap15-6 153.9 250 38.5
7 HUES9 p39 Control TeSR Ap15-7 183.5 250 45.9
8 HUES9 p39 ACTIVIN Ap15-8 149.3 250 37.3
9 HUES9 p39 BMP-4 Ap15-9 111.2 250 27.8
10 HUES3 p36 Control TeSR Ap15-10 153.5 250 38.4
11 HUES3 p36 ACTIVIN Ap15-11 87.5 250 21.9
12 HUES3 p36 BMP-4 Ap15-12 109.7 250 27.4
13 H1 p54 Control TeSR Ap15-13 191.3 250 47.8
14 H1 p54 ACTIVIN Ap15-14 108.7 250 27.2
15 ASThiPS4F5 p16 Control TeSR Ap15-15 134.2 250 33.6
*16 ASThiPS4F5 p16 ACTIVIN Ap15-16 50.2 500 25.1
17 ASThiPS4F4 p36 Control TeSR Ap15-17 183.0 250 45.8
18 ASThiPS4F4 p36 ACTIVIN Ap15-18 82.6 250 20.7
19 ASThiPS4F4 p36 BMP-4 Ap15-19 263.4 250 65.9
20 H1 p48 Ma15-2 215.2 200 43.0
21 H9 p47 Ma15-3 252.0 200 50.4
22 HUES3 p33 Ma15-4 226.1 200 45.2
23 HUES6 p26 Ma15-5 238.7 200 47.7
24 HUES8 p35 Ma15-6 83.4 200 16.7
25 HUES9 p36 Ma15-7 236.8 200 47.4
26 ASThiPS4F3 p24 (derived from Astrocytes) Ma15-8 175.9 200 35.2
27 ASThiPS4F4 p16 (derived from Astrocytes) Ma15-9 164.3 200 32.9
28 FiPS4F2 p32 (derived from IMR90 fibroblasts) Ma15-10 205.9 200 41.2
29 FiPS4F5 p29 (derived from IMR90 fibroblasts) Ma15-11 97.6 200 19.5
30 KiPS4FA p54 (derived from keratinocytes) Ma15-12 129.5 200 25.9
31 Huv-iPS4F6 p23 (derived from HUVECs) Ma15-13 263.1 200 52.6
32 IMR90 p6 Ma15-14 76.1 200 15.2
33 Keratinocytes p3 Ma15-15 138.7 200 27.7
34 H1 p47 Control Ma15-16 216.4 200 43.3
35 H1 p47 BMP-4 Ma15-17 137.6 200 27.5
36 ASThiPS4F5 p12 Control Ma15-18 224.6 200 44.9
37 ASThiPS4F5 p12 BMP-4 Ma15-19 111.7 200 22.3
38 Huv-iPS4F6 p23 Control Ma15-20 289.9 200 58.0
39 Huv-iPS4F6 p23 BMP-4 Ma15-21 172.9 200 34.6
40 Huv-iPS4F6 p23 ACTIVIN Ma15-1 86.7 250 21.7

Old gDNA of samples have been captured in the previous experiments

  • Note:
    • Some of gDNA concentrations were re-measured and note that some of them have conc. lower than 50ng/ul, and some of them don't have enough amount for bisulfite conversion
    • Samples KERAT MMTA and KiPS4F #8 have high conc., but very little volume. I added ~17ul H2O then re-measured conc. again
    • ***I may not do bis-cvt on the two samples, SR-21 and SR22 since the amount of gDNA was too little
No Sample IDs Lab IDs Conc. (ng/ul)
1 H9 p43 mTeSR SR-1 62.8
2 H9 p43 ACTIVIN SR-2 90.8
3 H9 p43 BMP4 SR-3 93.7
4 HUV0iPS4F1 p19 SR-4 98.0
5 HUV-hiPS4F1 p20 ACTIVIN SR-5 84.8
6 HUV-hiPS4F1 p20 BMP4 SR-6 78.6
7 HUV-iPS4F3 F19 SR-7 96.9
8 HUV-hiPS4F3 p20 ACTIVIN SR-8 91.9
9 HUV-hiPS4F3 p20 BMP4 SR-9 80.6
10 aMSCs p7 SR-10 184.8
11 MSCiPS #8 p14 (~4 ul) SR-11 105.4
12 MSCiPS #4 p14 SR-12 181.4
13 hNiPS2F p23 SR-13 270.0
14 hKiPS3F-7 p24 (~15ul) SR-14 33.0
15 hNSC SR-15 67.0
16 hFiPS4F-7 p9 SR-16 145.6
17 HUVEC p2 SR-17 245.3
18 hiPS AST4F-4 p9 SR-18 89.9
19 KERAT MMTA SR-19 159.5
20 KiPS4F #8 SR-20 195.7
21 ***olf MSC 19.1ng/ul volume only 3-5ul SR-21 19.1
22 ***KiPS 14ng/ul volume only 3-5ul SR-22 14.0

8 samples of bis-cvt gDNA from Dinh

  • The list will be filled later and need to re-measure of the conc. with ssDNA Qubit assay