Revision as of 16:03, 26 August 2012 by >Noi
DMR 330k BSPP capture of samples from Sergio[edit]
Sample list[edit]
on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-17
No.
|
Sample ID
|
Lab ID
|
Conc. (ng/ul)
|
Volume (ul)
|
Yields (ug)
|
1 |
HUES2 p41 |
Ap15-1 |
110.0 |
250 |
27.5
|
2 |
ASThiPS4F1 p12 |
Ap15-2 |
139.1 |
250 |
34.8
|
3 |
ASThiPS4F2 p12 |
Ap15-3 |
145.1 |
250 |
36.3
|
4 |
HUES6 p39 Control TeSR |
Ap15-4 |
295.5 |
250 |
73.9
|
5 |
HUES6 p39 ACTIVIN |
Ap15-5 |
128.1 |
250 |
32.0
|
6 |
HUES6 p39 BMP-4 |
Ap15-6 |
153.9 |
250 |
38.5
|
7 |
HUES9 p39 Control TeSR |
Ap15-7 |
183.5 |
250 |
45.9
|
8 |
HUES9 p39 ACTIVIN |
Ap15-8 |
149.3 |
250 |
37.3
|
9 |
HUES9 p39 BMP-4 |
Ap15-9 |
111.2 |
250 |
27.8
|
10 |
HUES3 p36 Control TeSR |
Ap15-10 |
153.5 |
250 |
38.4
|
11 |
HUES3 p36 ACTIVIN |
Ap15-11 |
87.5 |
250 |
21.9
|
12 |
HUES3 p36 BMP-4 |
Ap15-12 |
109.7 |
250 |
27.4
|
13 |
H1 p54 Control TeSR |
Ap15-13 |
191.3 |
250 |
47.8
|
14 |
H1 p54 ACTIVIN |
Ap15-14 |
108.7 |
250 |
27.2
|
15 |
ASThiPS4F5 p16 Control TeSR |
Ap15-15 |
134.2 |
250 |
33.6
|
*16 |
ASThiPS4F5 p16 ACTIVIN |
Ap15-16 |
50.2 |
500 |
25.1
|
17 |
ASThiPS4F4 p36 Control TeSR |
Ap15-17 |
183.0 |
250 |
45.8
|
18 |
ASThiPS4F4 p36 ACTIVIN |
Ap15-18 |
82.6 |
250 |
20.7
|
19 |
ASThiPS4F4 p36 BMP-4 |
Ap15-19 |
263.4 |
250 |
65.9
|
20 |
H1 p48 |
Ma15-2 |
215.2 |
200 |
43.0
|
21 |
H9 p47 |
Ma15-3 |
252.0 |
200 |
50.4
|
22 |
HUES3 p33 |
Ma15-4 |
226.1 |
200 |
45.2
|
23 |
HUES6 p26 |
Ma15-5 |
238.7 |
200 |
47.7
|
24 |
HUES8 p35 |
Ma15-6 |
83.4 |
200 |
16.7
|
25 |
HUES9 p36 |
Ma15-7 |
236.8 |
200 |
47.4
|
26 |
ASThiPS4F3 p24 (derived from Astrocytes) |
Ma15-8 |
175.9 |
200 |
35.2
|
27 |
ASThiPS4F4 p16 (derived from Astrocytes) |
Ma15-9 |
164.3 |
200 |
32.9
|
28 |
FiPS4F2 p32 (derived from IMR90 fibroblasts) |
Ma15-10 |
205.9 |
200 |
41.2
|
29 |
FiPS4F5 p29 (derived from IMR90 fibroblasts) |
Ma15-11 |
97.6 |
200 |
19.5
|
30 |
KiPS4FA p54 (derived from keratinocytes) |
Ma15-12 |
129.5 |
200 |
25.9
|
31 |
Huv-iPS4F6 p23 (derived from HUVECs) |
Ma15-13 |
263.1 |
200 |
52.6
|
32 |
IMR90 p6 |
Ma15-14 |
76.1 |
200 |
15.2
|
33 |
Keratinocytes p3 |
Ma15-15 |
138.7 |
200 |
27.7
|
34 |
H1 p47 Control |
Ma15-16 |
216.4 |
200 |
43.3
|
35 |
H1 p47 BMP-4 |
Ma15-17 |
137.6 |
200 |
27.5
|
36 |
ASThiPS4F5 p12 Control |
Ma15-18 |
224.6 |
200 |
44.9
|
37 |
ASThiPS4F5 p12 BMP-4 |
Ma15-19 |
111.7 |
200 |
22.3
|
38 |
Huv-iPS4F6 p23 Control |
Ma15-20 |
289.9 |
200 |
58.0
|
39 |
Huv-iPS4F6 p23 BMP-4 |
Ma15-21 |
172.9 |
200 |
34.6
|
40 |
Huv-iPS4F6 p23 ACTIVIN |
Ma15-1 |
86.7 |
250 |
21.7
|
Old gDNA of samples have been captured in the previous experiments[edit]
- Note:
- Some of gDNA concentrations were re-measured and note that some of them have conc. lower than 50ng/ul, and some of them don't have enough amount for bisulfite conversion
- Samples KERAT MMTA and KiPS4F #8 have high conc., but very little volume. I added ~17ul H2O then re-measured conc. again
- ***I may not do bis-cvt on the two samples, SR-21 and SR22 since the amount of gDNA was too little
No
|
Sample IDs
|
Lab IDs
|
Conc. (ng/ul)
|
1 |
H9 p43 mTeSR |
SR-1 |
62.8
|
2 |
H9 p43 ACTIVIN |
SR-2 |
90.8
|
3 |
H9 p43 BMP4 |
SR-3 |
93.7
|
4 |
HUV0iPS4F1 p19 |
SR-4 |
98.0
|
5 |
HUV-hiPS4F1 p20 ACTIVIN |
SR-5 |
84.8
|
6 |
HUV-hiPS4F1 p20 BMP4 |
SR-6 |
78.6
|
7 |
HUV-iPS4F3 F19 |
SR-7 |
96.9
|
8 |
HUV-hiPS4F3 p20 ACTIVIN |
SR-8 |
91.9
|
9 |
HUV-hiPS4F3 p20 BMP4 |
SR-9 |
80.6
|
10 |
aMSCs p7 |
SR-10 |
184.8
|
11 |
MSCiPS #8 p14 (~4 ul) |
SR-11 |
105.4
|
12 |
MSCiPS #4 p14 |
SR-12 |
181.4
|
13 |
hNiPS2F p23 |
SR-13 |
270.0
|
14 |
hKiPS3F-7 p24 (~15ul) |
SR-14 |
33.0
|
15 |
hNSC |
SR-15 |
67.0
|
16 |
hFiPS4F-7 p9 |
SR-16 |
145.6
|
17 |
HUVEC p2 |
SR-17 |
245.3
|
18 |
hiPS AST4F-4 p9 |
SR-18 |
89.9
|
19 |
KERAT MMTA |
SR-19 |
159.5
|
20 |
KiPS4F #8 |
SR-20 |
195.7
|
21 |
***olf MSC 19.1ng/ul volume only 3-5ul |
SR-21 |
19.1
|
22 |
***KiPS 14ng/ul volume only 3-5ul |
SR-22 |
14.0
|
8 samples of bis-cvt gDNA from Dinh[edit]
- The list will be filled later and need to re-measure of the conc. with ssDNA Qubit assay
Bisulfite conversion[edit]
Reagent prep
Add the following reagents to CT Conversion Reagent
- H2O 9.0mL
- M-Dissolving Buffer 500ul
- M-Dilution Buffer 3.0mL
Mix at room temperature with frequent vortexing or shaking for 15 minutes
Add 144 ml of 100% ethanol to the 36 ml M-Wash Buffer concentrate before use
Lab IDs
|
Conc. (ng/ul)
|
Volume for 1200ng
|
LabID ID
|
Well IDs
|
H2O
|
Total amount (ng)
|
CT conversion reagent (ul)
|
Total volume (ul)
|
Ap15-1 |
110.0 |
10.91 |
Ap15-1 |
A1 |
9.09 |
1200.00 |
130.00 |
150.00
|
Ap15-2 |
139.1 |
8.63 |
Ap15-2 |
B1 |
11.37 |
1200.00 |
130.00 |
150.00
|
Ap15-3 |
145.1 |
8.27 |
Ap15-3 |
C1 |
11.73 |
1200.00 |
130.00 |
150.00
|
Ap15-4 |
295.5 |
4.06 |
Ap15-4 |
D1 |
15.94 |
1200.00 |
130.00 |
150.00
|
Ap15-5 |
128.1 |
9.37 |
Ap15-5 |
E1 |
10.63 |
1200.00 |
130.00 |
150.00
|
Ap15-6 |
153.9 |
7.80 |
Ap15-6 |
F1 |
12.20 |
1200.00 |
130.00 |
150.00
|
Ap15-7 |
183.5 |
6.54 |
Ap15-7 |
G1 |
13.46 |
1200.00 |
130.00 |
150.00
|
Ap15-8 |
149.3 |
8.04 |
Ap15-8 |
H1 |
11.96 |
1200.00 |
130.00 |
150.00
|
Ap15-9 |
111.2 |
10.79 |
Ap15-9 |
A2 |
9.21 |
1200.00 |
130.00 |
150.00
|
Ap15-10 |
153.5 |
7.82 |
Ap15-10 |
B2 |
12.18 |
1200.00 |
130.00 |
150.00
|
Ap15-11 |
87.5 |
13.71 |
Ap15-11 |
C2 |
6.29 |
1200.00 |
130.00 |
150.00
|
Ap15-12 |
109.7 |
10.94 |
Ap15-12 |
D2 |
9.06 |
1200.00 |
130.00 |
150.00
|
Ap15-13 |
191.3 |
6.27 |
Ap15-13 |
E2 |
13.73 |
1200.00 |
130.00 |
150.00
|
Ap15-14 |
108.7 |
11.04 |
Ap15-14 |
F2 |
8.96 |
1200.00 |
130.00 |
150.00
|
Ap15-15 |
134.2 |
8.94 |
Ap15-15 |
G2 |
11.06 |
1200.00 |
130.00 |
150.00
|
Ap15-16 |
50.2 |
20.00 |
Ap15-16 |
H2 |
0.00 |
1200.00 |
130.00 |
150.00
|
Ap15-17 |
183.0 |
6.56 |
Ap15-17 |
A3 |
13.44 |
1200.00 |
130.00 |
150.00
|
Ap15-18 |
82.6 |
14.53 |
Ap15-18 |
B3 |
5.47 |
1200.00 |
130.00 |
150.00
|
Ap15-19 |
263.4 |
4.56 |
Ap15-19 |
C3 |
15.44 |
1200.00 |
130.00 |
150.00
|
Ma15-1 |
86.7 |
13.84 |
Ma15-1 |
D3 |
6.16 |
1200.00 |
130.00 |
150.00
|
Ma15-2 |
215.2 |
5.58 |
Ma15-2 |
E3 |
14.42 |
1200.00 |
130.00 |
150.00
|
Ma15-3 |
252.0 |
4.76 |
Ma15-3 |
F3 |
15.24 |
1200.00 |
130.00 |
150.00
|
Ma15-4 |
226.1 |
5.31 |
Ma15-4 |
G3 |
14.69 |
1200.00 |
130.00 |
150.00
|
Ma15-5 |
238.7 |
5.03 |
Ma15-5 |
H3 |
14.97 |
1200.00 |
130.00 |
150.00
|
Ma15-6 |
83.4 |
14.39 |
Ma15-6 |
A4 |
5.61 |
1200.00 |
130.00 |
150.00
|
Ma15-7 |
236.8 |
5.07 |
Ma15-7 |
B4 |
14.93 |
1200.00 |
130.00 |
150.00
|
Ma15-8 |
175.9 |
6.82 |
Ma15-8 |
C4 |
13.18 |
1200.00 |
130.00 |
150.00
|
Ma15-9 |
164.3 |
7.30 |
Ma15-9 |
D4 |
12.70 |
1200.00 |
130.00 |
150.00
|
Ma15-10 |
205.9 |
5.83 |
Ma15-10 |
E4 |
14.17 |
1200.00 |
130.00 |
150.00
|
Ma15-11 |
97.6 |
12.30 |
Ma15-11 |
F4 |
7.70 |
1200.00 |
130.00 |
150.00
|
Ma15-12 |
129.5 |
9.27 |
Ma15-12 |
G4 |
10.73 |
1200.00 |
130.00 |
150.00
|
Ma15-13 |
263.1 |
4.56 |
Ma15-13 |
H4 |
15.44 |
1200.00 |
130.00 |
150.00
|
Ma15-14 |
76.1 |
15.77 |
Ma15-14 |
A5 |
4.23 |
1200.00 |
130.00 |
150.00
|
Ma15-15 |
138.7 |
8.65 |
Ma15-15 |
B5 |
11.35 |
1200.00 |
130.00 |
150.00
|
Ma15-16 |
216.4 |
5.55 |
Ma15-16 |
C5 |
14.45 |
1200.00 |
130.00 |
150.00
|
Ma15-17 |
137.6 |
8.72 |
Ma15-17 |
D5 |
11.28 |
1200.00 |
130.00 |
150.00
|
Ma15-18 |
224.6 |
5.34 |
Ma15-18 |
E5 |
14.66 |
1200.00 |
130.00 |
150.00
|
Ma15-19 |
111.7 |
10.74 |
Ma15-19 |
F5 |
9.26 |
1200.00 |
130.00 |
150.00
|
Ma15-20 |
289.9 |
4.14 |
Ma15-20 |
G5 |
15.86 |
1200.00 |
130.00 |
150.00
|
Ma15-21 |
172.9 |
6.94 |
Ma15-21 |
H5 |
13.06 |
1200.00 |
130.00 |
150.00
|
Lab IDs
|
Conc. (ng/ul)
|
Volume for 1200ng
|
LabID ID
|
Well IDs
|
H2O
|
Total amount (ng)
|
CT conversion reagent (ul)
|
Total volume (ul)
|
SR-1 |
62.8 |
19.11 |
SR-1 |
A6 |
0.89 |
1200.00 |
130.00 |
150.00
|
SR-2 |
90.8 |
13.22 |
SR-2 |
B6 |
6.78 |
1200.00 |
130.00 |
150.00
|
SR-3 |
93.7 |
12.81 |
SR-3 |
C6 |
7.19 |
1200.00 |
130.00 |
150.00
|
SR-4 |
98.0 |
12.24 |
SR-4 |
D6 |
7.76 |
1200.00 |
130.00 |
150.00
|
SR-5 |
84.8 |
14.15 |
SR-5 |
E6 |
5.85 |
1200.00 |
130.00 |
150.00
|
SR-6 |
78.6 |
15.27 |
SR-6 |
F6 |
4.73 |
1200.00 |
130.00 |
150.00
|
SR-7 |
96.9 |
12.38 |
SR-7 |
G6 |
7.62 |
1200.00 |
130.00 |
150.00
|
SR-8 |
91.9 |
13.06 |
SR-8 |
H6 |
6.94 |
1200.00 |
130.00 |
150.00
|
SR-9 |
80.6 |
14.89 |
SR-9 |
A7 |
5.11 |
1200.00 |
130.00 |
150.00
|
SR-10 |
184.8 |
6.49 |
SR-10 |
B7 |
13.51 |
1200.00 |
130.00 |
150.00
|
SR-11 |
105.4 |
5.00 |
SR-11 |
C7 |
15.00 |
527.00 |
130.00 |
150.00
|
SR-12 |
181.4 |
6.62 |
SR-12 |
D7 |
13.38 |
1200.00 |
130.00 |
150.00
|
SR-13 |
270.0 |
4.44 |
SR-13 |
E7 |
15.56 |
1200.00 |
130.00 |
150.00
|
SR-14 |
33.0 |
20.00 |
SR-14 |
F7 |
0.00 |
495.00 |
130.00 |
150.00
|
SR-15 |
67.0 |
17.91 |
SR-15 |
G7 |
2.09 |
1200.00 |
130.00 |
150.00
|
SR-16 |
145.6 |
8.24 |
SR-16 |
H7 |
11.76 |
1200.00 |
130.00 |
150.00
|
SR-17 |
245.3 |
4.89 |
SR-17 |
A8 |
15.11 |
1200.00 |
130.00 |
150.00
|
SR-18 |
89.9 |
13.35 |
SR-18 |
B8 |
6.65 |
1200.00 |
130.00 |
150.00
|
SR-19 |
159.5 |
7.52 |
SR-19 |
C8 |
12.48 |
1200.00 |
130.00 |
150.00
|
SR-20 |
195.7 |
6.13 |
SR-20 |
D8 |
13.87 |
1200.00 |
130.00 |
150.00
|
SR-21 |
19.1 |
5.00 |
SR-21 |
E8 |
15.00 |
95.50 |
130.00 |
150.00
|
SR-22 |
14.0 |
5.00 |
SR-22 |
F8 |
15.00 |
70.00 |
130.00 |
150.00
|
Program
98°C for 10 minutes
64°C for 2.5 hours
4°C storage up to 20 hours
Measure conc. with ssDNA Qubit assay (used 1ul of bis-cvt gDNA)
Qubit ssDNA assay[edit]
- Sample in assay 1ul, dilution 200x
Sample
|
LabID ID
|
Well IDs
|
Concentration in the Qubit
|
'
|
Final conc. (ng/ul)
|
Yields in 16ul (ng)
|
LabID ID
|
Well IDs
|
Repeated samples
|
1.00 |
Ap15-1 |
A1 |
57.00 |
ng/mL |
11.40 |
182.40 |
Ap15-1 |
A1 |
X
|
2.00 |
Ap15-2 |
B1 |
62.20 |
ng/mL |
12.44 |
199.04 |
Ap15-2 |
B1 |
X
|
3.00 |
Ap15-3 |
C1 |
81.70 |
ng/mL |
16.34 |
261.44 |
Ap15-3 |
C1 |
X
|
4.00 |
Ap15-4 |
D1 |
75.40 |
ng/mL |
15.08 |
241.28 |
Ap15-4 |
D1 |
X
|
5.00 |
Ap15-5 |
E1 |
59.60 |
ng/mL |
11.92 |
190.72 |
Ap15-5 |
E1 |
X
|
6.00 |
Ap15-6 |
F1 |
74.50 |
ng/mL |
14.90 |
238.40 |
Ap15-6 |
F1 |
X
|
7.00 |
Ap15-7 |
G1 |
76.40 |
ng/mL |
15.28 |
244.48 |
Ap15-7 |
G1 |
X
|
8.00 |
Ap15-8 |
H1 |
76.40 |
ng/mL |
15.28 |
244.48 |
Ap15-8 |
H1 |
X
|
9.00 |
Ap15-9 |
A2 |
76.10 |
ng/mL |
15.22 |
243.52 |
Ap15-9 |
A2 |
X
|
10.00 |
Ap15-10 |
B2 |
60.10 |
ng/mL |
12.02 |
192.32 |
Ap15-10 |
B2 |
X
|
11.00 |
Ap15-11 |
C2 |
56.40 |
ng/mL |
11.28 |
180.48 |
Ap15-11 |
C2 |
X
|
12.00 |
Ap15-12 |
D2 |
59.00 |
ng/mL |
11.80 |
188.80 |
Ap15-12 |
D2 |
X
|
13.00 |
Ap15-13 |
E2 |
72.50 |
ng/mL |
14.50 |
232.00 |
Ap15-13 |
E2 |
X
|
14.00 |
Ap15-14 |
F2 |
74.30 |
ng/mL |
14.86 |
237.76 |
Ap15-14 |
F2 |
X
|
15.00 |
Ap15-15 |
G2 |
58.90 |
ng/mL |
11.78 |
188.48 |
Ap15-15 |
G2 |
X
|
16.00 |
Ap15-16 |
H2 |
45.10 |
ng/mL |
9.02 |
144.32 |
Ap15-16 |
H2 |
X
|
17.00 |
Ap15-17 |
A3 |
76.40 |
ng/mL |
15.28 |
244.48 |
Ap15-17 |
A3 |
X
|
18.00 |
Ap15-18 |
B3 |
54.90 |
ng/mL |
10.98 |
175.68 |
Ap15-18 |
B3 |
X
|
19.00 |
Ap15-19 |
C3 |
78.90 |
ng/mL |
15.78 |
252.48 |
Ap15-19 |
C3 |
X
|
20.00 |
Ma15-1 |
D3 |
44.10 |
ng/mL |
8.82 |
141.12 |
Ma15-1 |
D3 |
X
|
21.00 |
Ma15-2 |
E3 |
49.80 |
ng/mL |
9.96 |
159.36 |
Ma15-2 |
E3 |
X
|
22.00 |
Ma15-3 |
F3 |
51.60 |
ng/mL |
10.32 |
165.12 |
Ma15-3 |
F3 |
X
|
23.00 |
Ma15-4 |
G3 |
58.20 |
ng/mL |
11.64 |
186.24 |
Ma15-4 |
G3 |
X
|
24.00 |
Ma15-5 |
H3 |
60.10 |
ng/mL |
12.02 |
192.32 |
Ma15-5 |
H3 |
X
|
25.00 |
Ma15-6 |
A4 |
40.60 |
ng/mL |
8.12 |
129.92 |
Ma15-6 |
A4 |
X
|
26.00 |
Ma15-7 |
B4 |
59.90 |
ng/mL |
11.98 |
191.68 |
Ma15-7 |
B4 |
X
|
27.00 |
Ma15-8 |
C4 |
54.50 |
ng/mL |
10.90 |
174.40 |
Ma15-8 |
C4 |
X
|
28.00 |
Ma15-9 |
D4 |
43.60 |
ng/mL |
8.72 |
139.52 |
Ma15-9 |
D4 |
X
|
29.00 |
Ma15-10 |
E4 |
73.10 |
ng/mL |
14.62 |
233.92 |
Ma15-10 |
E4 |
X
|
30.00 |
Ma15-11 |
F4 |
49.70 |
ng/mL |
9.94 |
159.04 |
Ma15-11 |
F4 |
X
|
31.00 |
Ma15-12 |
G4 |
36.90 |
ng/mL |
7.38 |
118.08 |
Ma15-12 |
G4 |
X
|
32.00 |
Ma15-13 |
H4 |
60.00 |
ng/mL |
12.00 |
192.00 |
Ma15-13 |
H4 |
X
|
33.00 |
Ma15-14 |
A5 |
28.00 |
ng/mL |
5.60 |
89.60 |
Ma15-14 |
A5 |
X
|
34.00 |
Ma15-15 |
B5 |
27.70 |
ng/mL |
5.54 |
88.64 |
Ma15-15 |
B5 |
X
|
35.00 |
Ma15-16 |
C5 |
53.50 |
ng/mL |
10.70 |
171.20 |
Ma15-16 |
C5 |
X
|
36.00 |
Ma15-17 |
D5 |
51.10 |
ng/mL |
10.22 |
163.52 |
Ma15-17 |
D5 |
X
|
37.00 |
Ma15-18 |
E5 |
53.60 |
ng/mL |
10.72 |
171.52 |
Ma15-18 |
E5 |
X
|
38.00 |
Ma15-19 |
F5 |
51.40 |
ng/mL |
10.28 |
164.48 |
Ma15-19 |
F5 |
X
|
39.00 |
Ma15-20 |
G5 |
48.90 |
ng/mL |
9.78 |
156.48 |
Ma15-20 |
G5 |
X
|
40.00 |
Ma15-21 |
H5 |
55.40 |
ng/mL |
11.08 |
177.28 |
Ma15-21 |
H5 |
X
|
1.00 |
H9 p43 mTeSR |
SR-1 |
108.00 |
ng/mL |
21.60 |
345.60 |
SR-1 |
A6 |
-
|
2.00 |
H9 p43 ACTIVIN |
SR-2 |
64.90 |
ng/mL |
12.98 |
207.68 |
SR-2 |
B6 |
X
|
3.00 |
H9 p43 BMP4 |
SR-3 |
133.00 |
ng/mL |
26.60 |
425.60 |
SR-3 |
C6 |
-
|
4.00 |
HUV0iPS4F1 p19 |
SR-4 |
140.00 |
ng/mL |
28.00 |
448.00 |
SR-4 |
D6 |
-
|
5.00 |
HUV-hiPS4F1 p20 ACTIVIN |
SR-5 |
95.40 |
ng/mL |
19.08 |
305.28 |
SR-5 |
E6 |
-
|
6.00 |
HUV-hiPS4F1 p20 BMP4 |
SR-6 |
135.00 |
ng/mL |
27.00 |
432.00 |
SR-6 |
F6 |
-
|
7.00 |
HUV-iPS4F3 F19 |
SR-7 |
134.00 |
ng/mL |
26.80 |
428.80 |
SR-7 |
G6 |
-
|
8.00 |
HUV-hiPS4F3 p20 ACTIVIN |
SR-8 |
96.10 |
ng/mL |
19.22 |
307.52 |
SR-8 |
H6 |
-
|
9.00 |
HUV-hiPS4F3 p20 BMP4 |
SR-9 |
120.00 |
ng/mL |
24.00 |
384.00 |
SR-9 |
A7 |
-
|
10.00 |
aMSCs p7 |
SR-10 |
53.90 |
ng/mL |
10.78 |
172.48 |
SR-10 |
B7 |
X
|
11.00 |
MSCiPS #8 p14 (~4 ul) |
SR-11 |
71.60 |
ng/mL |
14.32 |
229.12 |
SR-11 |
C7 |
Can\'t repeat
|
12.00 |
MSCiPS #4 p14 |
SR-12 |
94.60 |
ng/mL |
18.92 |
302.72 |
SR-12 |
D7 |
-
|
13.00 |
hNiPS2F p23 |
SR-13 |
29.60 |
ng/mL |
5.92 |
94.72 |
SR-13 |
E7 |
X
|
14.00 |
hKiPS3F-7 p24 (~15ul) |
SR-14 |
7.13 |
ng/mL |
1.43 |
22.82 |
SR-14 |
F7 |
X
|
15.00 |
hNSC |
SR-15 |
70.90 |
ng/mL |
14.18 |
226.88 |
SR-15 |
G7 |
X
|
16.00 |
hFiPS4F-7 p9 |
SR-16 |
23.10 |
ng/mL |
4.62 |
73.92 |
SR-16 |
H7 |
X
|
17.00 |
HUVEC p2 |
SR-17 |
50.80 |
ng/mL |
10.16 |
162.56 |
SR-17 |
A8 |
X
|
18.00 |
hiPS AST4F-4 p9 |
SR-18 |
131.00 |
ng/mL |
26.20 |
419.20 |
SR-18 |
B8 |
|
19.00 |
KERAT MMTA |
SR-19 |
19.80 |
ng/mL |
3.96 |
63.36 |
SR-19 |
C8 |
X
|
20.00 |
KiPS4F #8 |
SR-20 |
19.00 |
ng/mL |
3.80 |
60.80 |
SR-20 |
D8 |
X
|
21.00 |
olf MSC 19.1ng/ul volume only 3-5ul |
SR-21 |
4.60 |
ng/mL |
0.92 |
14.72 |
SR-21 |
E8 |
can\'t repeat
|
22.00 |
KiPS 14ng/ul volume only 3-5ul |
SR-22 |
7.42 |
ng/mL |
1.48 |
23.74 |
SR-22 |
F8 |
can\'t repeat
|
- From the yields of bis-cvt gDNA, many samples have the yields lower than 300ng or 200ng which is the amount the acceptable for capture with BSPP. I checked the yields of the old samples I have processed before (SR-1 - SR-9) to see if this is the technical error of this batch. I got the total yields of SR-1 -15 pretty much the same as I got before. This suggested that there are not the batch effect, but may be the quality of gDNA. I need to repeat bis-cvt again to get enough yield for capture. However, there are some sample that has no more gDNA or has very low concentration, and I don't expected to get higher yield from those samples.