Tina:LabNotes/2012-6-1

From ZhangLabWiki
Revision as of 01:12, 2 June 2012 by >TinaLo (Created page with "*Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday). *Passage P8 InsACGGsAlov3 cel...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search
  • Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday).
  • Passage P8 InsACGGsAlov3 cells into P9 (one well on MEF; one well feeder free)
  • Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank.
  • Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information.
  • Transfer LIF from -80 to -20.