Tina:LabNotes/2012-6-1
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- Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday).
- Passage P8 InsACGGsAlov3 cells into P9 (one well on MEF; one well feeder free)
- Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank.
- Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information.
- Transfer LIF from -80 to -20.