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Component | Volume |
Knockout DMEM | 409.5 mL |
ESC qualified FBS (heat inactivated) | 75 mL |
Non-essential amino acids (NEAA) | 5 mL |
Glutamax | 5 mL |
Penicillin / Streptomycin | 5 mL |
1000X BME soulution | 500 uL |
ESGRO LIF | 50 uL |
Total | 500 mL |
border=1 Component Volume Knockout DMEM 409.5 mL ESC qualified FBS (heat inactivated) 75 mL Non-essential amino acids (NEAA) 5 mL Glutamax 5 mL Penicillin / Streptomycin 5 mL 1000X BME soulution 500 uL ESGRO LIF 50 uL Total 500 mL