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Component Volume
Knockout DMEM 409.5 mL
ESC qualified FBS (heat inactivated) 75 mL
Non-essential amino acids (NEAA) 5 mL
Glutamax 5 mL
Penicillin / Streptomycin 5 mL
1000X BME soulution 500 uL
ESGRO LIF 50 uL
Total 500 mL

border=1 Component Volume Knockout DMEM 409.5 mL ESC qualified FBS (heat inactivated) 75 mL Non-essential amino acids (NEAA) 5 mL Glutamax 5 mL Penicillin / Streptomycin 5 mL 1000X BME soulution 500 uL ESGRO LIF 50 uL Total 500 mL