Dinh:COMPUTATIONAL/bisReadMapper
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To perform bisulfite reads mapping with bisReadMapper
- Know where the following files and software are:
1) Reference index 2) soap or bowtie2 currently I have bowtie2 running with --fast setting, which seems to be a lot slower than soap. this setting may be changed to --very-fast in the future for mapping BSPP data. 3) samtools 4) reads (do not need to copy the reads to the current directory, nor concatenate them)
- Master shell script: 1 - perform mapping.
We need to perform mapping and make sure that all reads mapped properly.
- This master script process bisulfite reads.
My_ref="/projects/zhang-lab/ddiep/bisHg19"
- Remember to use the correct number of nodes! Don't use too many nodes because this is slow
- Remember to set the correct temporary directory for sorting in this script!
bisReadMapper="/home/ddiep/scripts/MethylationPipeline/bisReadMapper_Bowtie2.pl"
for f in list_* do
g=`echo $f | sed 's/list_//g'` /home/ddiep/scripts/MethylationPipeline/wBOWTIE/submitBSPPMapJobs.pl $g $My_ref $bisReadMapper < $f
done
- now we need to check that all jobs finished without error.