Noi/NOTES/2012-7-12
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epMotion test (AMPure bead purification)
- Things need to adjust
- Speed of aspiration and dispension of PCR product
- Avoid to reuse the tips to pipette the same reagent like EtOH in washing step. This need to adjust protocol to be a little more complicated in fill in the sample number in each step.
Plan of epMotion testing (two round AMPure bead purification and vary amount of start DNA amount
- DNA amount range (in 80ul of the liquid)
- 5ng
- 10ng
- 25ng
- 50ng
- 100ng
- 200ng
- The pooled PCR product from Sergio's samples is approximately 22ng/ul by PAGE quantification
DNA amount (ng) | Total volume (ul) | Conc (ng/ul) | DNA (stock 22ng/ul) | H2O | Total volume | DNA amount (ng) |
5 | 80 | 0.0625 | 2.84 | 997.16 | 1000.00 | 5 |
10 | 80 | 0.1250 | 5.68 | 994.32 | 1000.00 | 10 |
25 | 80 | 0.3125 | 14.20 | 985.80 | 1000.00 | 25 |
50 | 80 | 0.6250 | 28.41 | 971.59 | 1000.00 | 50 |
100 | 80 | 1.2500 | 56.82 | 943.18 | 1000.00 | 100 |
200 | 80 | 2.5000 | 113.64 | 886.36 | 1000.00 | 200 |