Noi/NOTES/2012-7-12

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Revision as of 00:10, 14 July 2012 by >Noi (Created page with "= epMotion test (AMPure bead purification) = * Things need to adjust ** Speed of aspiration and dispension of PCR product ** Avoid to reuse the tips to pipette the same reagen...")
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epMotion test (AMPure bead purification)

  • Things need to adjust
    • Speed of aspiration and dispension of PCR product
    • Avoid to reuse the tips to pipette the same reagent like EtOH in washing step. This need to adjust protocol to be a little more complicated in fill in the sample number in each step.

Plan of epMotion testing (two round AMPure bead purification and vary amount of start DNA amount

  • DNA amount range (in 80ul of the liquid)
    • 5ng
    • 10ng
    • 25ng
    • 50ng
    • 100ng
    • 200ng
  • The pooled PCR product from Sergio's samples is approximately 22ng/ul by PAGE quantification
DNA amount (ng) Total volume (ul) Conc (ng/ul) DNA (stock 22ng/ul) H2O Total volume DNA amount (ng)
5 80 0.0625 2.84 997.16 1000.00 5
10 80 0.1250 5.68 994.32 1000.00 10
25 80 0.3125 14.20 985.80 1000.00 25
50 80 0.6250 28.41 971.59 1000.00 50
100 80 1.2500 56.82 943.18 1000.00 100
200 80 2.5000 113.64 886.36 1000.00 200