Rui:LabNotes/SingleCell/2012-12-17

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Revision as of 01:31, 18 December 2012 by >RuiLiu (→‎Samples and procedure)
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Hs Ref RNA with totoRNAseq ranging from 1ng to 10pg

  • Repeat procedure used on 11.8.12/11.10.12
  • set 2 condition prior to RT: 72C 3min vs 10C 10min
  • set 2 condition for TSO: TSO.r02 vs TSO.idXX

Samples and procedure

  • PNK: 1:2d in water
  • PAP:1:4d in water
  • RNaseIn: 1:2d in water
  • MMLV:1:2d in water
' RNA Frag. T20.id ' Anneal after RT TSO N2.id
X2 1ng 1min 1uM id01-02 72C, 3min 1uM
X2 NTC 1min 1uM id07-08 72C, 3min 3.2ng TSO.r02 N2.id01
X12 100pg 1min 1uM others to 16 72C, 3min 1.6ng X 2 TSO.id02 N2.id02
X2 1ng 1min 1uM id01-02 10C,15min 1uM
X2 NTC 1min 1uM id07-08 10C,15min 3.2ng TSO.r02 N2.id03
X12 100pg 1min 1uM others to 16 10C,15min 1.6ng X 2 TSO.id04 N2.id04
X2 100pg 1min 0.1uM id01-02 72C, 3min 1uM
X2 NTC 1min 0.1uM id07-08 72C, 3min 320pg TSO.r02 N2.id05
X12 10pg 1min 0.1uM others to 16 72C, 3min 0.16ng X 2 TSO.id06 N2.id06
X2 100pg 1min 0.1uM id01-02 10C,15min 1uM
X2 NTC 1min 0.1uM id07-08 10C,15min 320pg TSO.r02 N2.id07
X12 10pg 1min 0.1uM others to 16 10C,15min 0.16ng X 2 TSO.id08 N2.id08