Rui:RNAseq analysis on 121226 HL140
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RNAseq on 121226_HL140
- Continue with Kun's note [1]
- A number of questions that I hope to answer with this data set include:
- Is there any indication that we can obtain reasonable data from 10pg of input total RNA?
- Is there a difference in efficiency between TSOs with and without the 8bp barcodes?
- Is there a difference in efficiency between T20V and T20VN primer?
- Is there a difference in efficiency between 72C and 10C annealing?
- Is NTC clean enough compared with 10pg samples?
Reads information
Input ng | # | N2.id01_avg | N2.id02_avg | N2.id03_avg | N2.id04_avg |
0 | 2 | 0.18% | 0.16% | 0.81% | 0.54% |
0.1 | 12 | 2.58% | 2.63% | 3.60% | 3.48% |
1 | 2 | 34.36% | 34.07% | 27.62% | 28.60% |
total Reads | 3,146,372 | 2,366,588 | 4,194,796 | 3,431,696 | |
Input ng | # | N2.id05_avg | N2.id06_avg | N2.id07_avg | N2.id08_avg |
0 | 2 | 0.70% | 0.84% | 3.47% | 3.53% |
0.01 | 12 | 2.76% | 2.81% | 5.26% | 5.24% |
0.1 | 2 | 32.73% | 32.32% | 14.95% | 15.03% |
total reads | 3,483,059 | 1,668,975 | 5,572,495 | 1,774,114 |