Rui:LabNotes/SingleCell/2013-1-15

From ZhangLabWiki
Revision as of 02:44, 16 January 2013 by >RuiLiu (Created page with "==totoRNAseq with 10ng/1ng Universal Ref. RNA== ===Samples=== {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

totoRNAseq with 10ng/1ng Universal Ref. RNA

Samples

' RNA Frag. Purification PNK PAP RT Beads TSO USER PCR_F PCR_R
1 10ng shearing \ yes yes T20.r01 yes TSO.r02 yes PB_PCR.F N2.01
2 SMARTer \ P1-STRT N2.02
3 1ng shearing \ yes yes T20.r01 yes TSO.r02 yes PB_PCR.F N2.03
4 10ng Mg2+ \ yes yes T20.r01 yes TSO.r02 yes PB_PCR.F N2.04
5 SMARTer \ P1-STRT N2.05
6 1ng Mg2+ \ yes yes T20.r01 yes TSO.r02 yes PB_PCR.F N2.06
7 10ng RNaseIII yes \ yes T20.r01 yes TSO.r02 yes PB_PCR.F N2.07
8 SMARTer \ P1-STRT N2.08
9 1ng RNaseIII yes \ yes T20.r01 yes TSO.r02 yes PB_PCR.F N2.09
10 10ng Mg2+ \ yes yes T20.r01 EtOH TSO.r02 yes PB_PCR.F N2.10
11 1ng Mg2+ \ yes yes T20.r01 EtOH TSO.r02 yes PB_PCR.F N2.11
12 10ng Mg2+ \ yes yes T20.r01/TSO.r02 yes PB_PCR.F N2.12
13 1ng Mg2+ \ yes yes T20.r01/TSO.r02 yes PB_PCR.F N2.13

Procedure

Results

File:1.15.13 QPCR s1-9.jpgFile:1.15.13 gel s1-9.jpg

File:1.15.13 QPCR s10-13.jpgFile:1.15.13 gel s10-13.jpg