Rui:LabNotes/SingleCell/2013-1-15
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totoRNAseq with 10ng/1ng Universal Ref. RNA
Samples
' | RNA | Frag. | Purification | PNK | PAP | RT | Beads | TSO | USER | PCR_F | PCR_R |
1 | 10ng | shearing | \ | yes | yes | T20.r01 | yes | TSO.r02 | yes | PB_PCR.F | N2.01 |
2 | SMARTer | \ | P1-STRT | N2.02 | |||||||
3 | 1ng | shearing | \ | yes | yes | T20.r01 | yes | TSO.r02 | yes | PB_PCR.F | N2.03 |
4 | 10ng | Mg2+ | \ | yes | yes | T20.r01 | yes | TSO.r02 | yes | PB_PCR.F | N2.04 |
5 | SMARTer | \ | P1-STRT | N2.05 | |||||||
6 | 1ng | Mg2+ | \ | yes | yes | T20.r01 | yes | TSO.r02 | yes | PB_PCR.F | N2.06 |
7 | 10ng | RNaseIII | yes | \ | yes | T20.r01 | yes | TSO.r02 | yes | PB_PCR.F | N2.07 |
8 | SMARTer | \ | P1-STRT | N2.08 | |||||||
9 | 1ng | RNaseIII | yes | \ | yes | T20.r01 | yes | TSO.r02 | yes | PB_PCR.F | N2.09 |
10 | 10ng | Mg2+ | \ | yes | yes | T20.r01 | EtOH | TSO.r02 | yes | PB_PCR.F | N2.10 |
11 | 1ng | Mg2+ | \ | yes | yes | T20.r01 | EtOH | TSO.r02 | yes | PB_PCR.F | N2.11 |
12 | 10ng | Mg2+ | \ | yes | yes | T20.r01/TSO.r02 | yes | PB_PCR.F | N2.12 | ||
13 | 1ng | Mg2+ | \ | yes | yes | T20.r01/TSO.r02 | yes | PB_PCR.F | N2.13 |