Leica SP8 Confocal Microscope

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Revision as of 21:19, 17 January 2013 by >Hosuki78
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Location

PFBH 036


Leica support contact

  1. Technical support: (Toll Free) 866-830-0735
  2. Clark Thom
    • West Coast Team Leader Field Service
    • 619-278-8324
    • clark.thom@leica-microsystems.com
  3. Carlos Alonso
    • Confocal Applications Specialist
    • 858-353-8062
    • carlos.alonso@leica-microsystems.com
  • (Call technical support first before trying to contact Clark)


General specification

  1. 3 Laser source – 488, 552, 638nm, Solid State, up to 20mW
  2. 2 PMT (Photo Multiplier Tube)
  3. 3 Objective Lens
    • 10x NA = 0.3
    • 20x NA = 0.75(Glycerine or Oil immersion)
    • 63x NA=1.4(Oil immersion)
  4. Z-axis step size – 50nm
  5. Scan area - 1.16 mm x 1.16mm (@10x obj., zoom = 1x) ~ 24.22 um x 24.22um (@10x obj., zoom = 48x)
  6. Image size - up to 8192 x 8192 pixel
    • 1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
    • Max. Scan field : 21.2mm (Tile scanning)


Sample preparation

  • Only one important consideration is that substrate must be #1.5 glass slide!

=Workstation Login

TCS User/True!Confocal55 (case sensitive)

Turning on/off sequence

  1. Turn on sequence
    • Confocal Power supply : Turn on from left to right Scan power --> Laser power --> Key (turn clockwise)
    • PC power strip : Turn on the switch on the strip
  2. Turn off sequence --> Reverse way of turn on sequence
    • Shut down PC
    • Turn off PC power strip
    • Turn off confocal power supply : Turn off from right to left : Key --> Laser power --> Scan power


File:ConfocalPower TurnOn.jpg File:ConfocalPower TurnOff.jpg


Summary of operating Manual

  • (will be added)