Hosuk:Cell fixation in culture dish

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Revision as of 19:06, 30 January 2013 by >Hosuki78
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  1. For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
  2. 1mL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
  3. Change media of fresh formalin+PBS (1mL), incubate for 15min at 37C,
  4. Wash with PBS, and add 70% Ethanol (2mL)
  5. Store at 4C