Hosuk:Cell fixation in culture dish
Jump to navigation
Jump to search
- For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
- 1mL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
- Change media of fresh formalin+PBS (1mL), incubate for 15min at 37C,
- Wash with PBS, and add 70% Ethanol (2mL)
- Store at 4C