Hosuk:Cell fixation in culture dish
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- For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
- NO aspirate media. add 200 ul formalin(~39% formaldehyde) to 2 ml media directly,
- incubate for 15 min at 37C
- aspirate
- quickly add pre warmed 10% formalin in PBS
- incubate at 37C for 15 min
- aspirate
- wash with PBS 3 times at RT
- incubate in 70% etoh for 1 min or store in 70% etoh up to a week at 4C