EricChu:LabNotesMDA/2013-2-8
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Verifying qPCR by 10,000 MEF cells MDA[edit]
Result: This time DNA was enough to create a standard curve
Running two sets of mouse specific primers (including msineB)[edit]
- Created standard for mouse genomic DNA and mouse MDA products with 1ng/ul, 100pg/ul, 10pg/ul and 1pg/ul at 56C extension.
- Measured MDA products (1/28) and (2/4) from devices
Result: the msineB did not work at 56C
Setting Gradient for msineB extension temperature[edit]
- Extension step was set from 58C-52C on PCR machine
- 100pg/ul MDA product and genomic mouse DNA was used
Result: the best temperature for msineB was 52.5C