EricChu:LabNotesMDA/2013-2-8

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Revision as of 23:03, 12 February 2013 by >Ericchu (Created page with "==Verifying qPCR by 10,000 MEF cells MDA== Result: This time DNA was enough to create a standard curve ==Running two sets of mouse specific primers (including msineB)== #C...")
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Verifying qPCR by 10,000 MEF cells MDA[edit]

Result: This time DNA was enough to create a standard curve


Running two sets of mouse specific primers (including msineB)[edit]

  1. Created standard for mouse genomic DNA and mouse MDA products with 1ng/ul, 100pg/ul, 10pg/ul and 1pg/ul at 56C extension.
  2. Measured MDA products (1/28) and (2/4) from devices

Result: the msineB did not work at 56C


Setting Gradient for msineB extension temperature[edit]

  1. Extension step was set from 58C-52C on PCR machine
  2. 100pg/ul MDA product and genomic mouse DNA was used

Result: the best temperature for msineB was 52.5C