Noi/NOTES/2013-3-14
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Components | 1rxn | 6.5 rxn |
Captured template | 12.00 | 0.00 |
10uM Forward no barcodes | 2.00 | 13.00 |
10uM Reverse +Indx | 2.00 | 0.00 |
2x KAPA SYBG fast MM | 50.00 | 325.00 |
H2O | 34.00 | 221.00 |
Total | 100.00 | 650.00 |
- Aliquot 86ul, add 2ul of reverse Ind, 12ul of circular DNA
Components | ' | ' |
Captured template | 1rxn | 6.5 rxn |
10uM Forward + Indx | 12.00 | 0.00 |
100uM Reverse no barcodes | 2.00 | 0.00 |
2x KAPA SYBG fast MM | 2.00 | 13.00 |
H2O | 50.00 | 325.00 |
Total | 34.00 | 221.00 |
100.00 | 650.00 |
- Aliquot 86ul, add 2ul of Forward Ind, 12ul of circular DNA