Tina:Cardiomyocyte differentiation in H9 ESCs (CDH9)

From ZhangLabWiki
Revision as of 01:29, 3 April 2013 by >TinaLo (Created page with "*'''Purpose''': To determine how Metallo Lab's modified cardiomyocyte differentiation protocol works on H9 ESCs. *'''Cells''': Brainbow H9 constructs ==Experiment #1== *Study...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search
  • Purpose: To determine how Metallo Lab's modified cardiomyocyte differentiation protocol works on H9 ESCs.
  • Cells: Brainbow H9 constructs

Experiment #1

  • Study Purpose: Try 3 different induction medium and 2 different cell density during differentiation and see how it works on H9 ESCs directed cardiomyocyte differentiation.
  • Date:
  • Cells:
  • Protocol used: Christian Metallo Lab protocol.
  • Three induction medium used: High Gln induction medium, Low Gln induction medium, DMK induction medium
  • Two cell density used: 100%, 80% confluency.
  • Experimental design:

12-well plate #1

100% confluency 1 2 3 4
A High Gln, repeat 1 High Gln, repeat 2 High Gln, repeat 3 High Gln, repeat 4
B Low Gln, repeat 1 Low Gln, repeat 2 Low Gln, repeat 3 Low Gln, repeat 4
C DMK, repeat 1 DMK, repeat 2 DMK, repeat 3 DMK, repeat 4

12-well plate #2

80% confluency 1 2 3 4
A High Gln, repeat 1 High Gln, repeat 2 High Gln, repeat 3 High Gln, repeat 4
B Low Gln, repeat 1 Low Gln, repeat 2 Low Gln, repeat 3 Low Gln, repeat 4
C DMK, repeat 1 DMK, repeat 2 DMK, repeat 3 DMK, repeat 4