Matt:LabNotes/2013-3-12

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Revision as of 17:40, 8 April 2013 by >Mzcai (Created page with "===cDNA + RNase continued=== *Used RNase A from Thermo Scientific *Added 20ul H20 to each cDNA sample to bring total volume to 100ul *Added 1.5ul RNase A (10ug/ul) *Incubated ...")
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cDNA + RNase continued[edit]

  • Used RNase A from Thermo Scientific
  • Added 20ul H20 to each cDNA sample to bring total volume to 100ul
  • Added 1.5ul RNase A (10ug/ul)
  • Incubated for 15 min at 37C
  • Purified cDNA with Qiagen minelute column
  • Qubit conc:

Probe Capture[edit]