Daniel Jacobsen

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Revision as of 17:27, 13 May 2017 by >Djacobse (→‎Protocols)
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Notebook[edit]

High Resolution Chromosome Painting

Genome Miner

Haplotyping

Methylation Deconvolution Project

RNA FISH Project

RNA FISH-FACS based sorting

Combo-Lock

Positional Tissue Sequencing

Protocols[edit]

Purification Protocols CERC Protocols Gel Protocols FISH Protocols Misc Protocols Combo-Lock Protocols
Qiaquick Column Purification Freeze Cells (CERC) Urea Gel Quantification FISH (Fluorescence in situ Hybridization) CPT-Seq Antibody-Oligo Linkage
ssDNA Column (Zymo) Splitting Cells Alexa Fluorophore Dye Coupling qPCR C Probe Hybridization
Centri-Sep Protocol (Princeton Separations) High MW DNA Extraction FISH on Tissue Sections Nuclear Adhesion to Slides Antibody Hybridization
Ethanol Precipitation RNA Scope PAGE Gel Creation on Slides Latch and Padlock Hybridization
Magnetic Beads Purification RNA FISH Cell Sorting Emulsion PCR Circularization/Rolling Circle Amplification


Amicon Ultra Centrifugal Filters Stellaris Probes Qubit Quantification

Cell Lines[edit]

NCI-h1975 U-87MG Cell Line T98-G BE2-C

Reagents[edit]

DpnII

Lambda Exonuclease

Lab Meeting Presentations[edit]

High Res Chromosome Painting

Freezer Boxes[edit]

Stored Probes

Templates[edit]

Gel Template

Probe Template

Training[edit]

Lab Safety/IIPP Training Certificate: File:Lab saftey jacobsen.pdf
Stem Cell Ethics Training Certificate: File:Stem Cell Ethics Jacobsen.pdf
Annual Hazards Safety Training Certificate: File:Jacobsen AnnualSafetyTraining2013.pdf
Laboratory Safety Fundamentals Training Certificate:  File:JacobsenLabSafetyFundamentals.pdf
Bloodborne Pathogens Training Certificate: File:JacobsenDaniel BBP Training.pdf