Daniel:Notebook/HiResChrPaint/2013-3-26
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Probe Set Prep (Started 3/26/2013)
Expansion PCR and Ethanol Precipitation
Expansion PCR
1. Set up 8 reactions for each of the 8 probes (64 reactions total)
per rxn | V4S1 | V4S2 | V4S3 | V4S4 | |
20 nM OligoPool | 0.2 | 1.7 | 1.7 | 1.7 | 1.7 |
2X Kapa SYBR qPCR MM | 50 | 425 | 425 | 425 | 425 |
100 uM AP1V41U | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
100 uM AP2V4 | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
2 mM aa-dUTP | 3 | 25.5 | 25.5 | 25.5 | 25.5 |
H20 | 46 | 391 | 391 | 391 | 391 |
Total Volume | 100 | 850 | 850 | 850 | 850 |
per rxn | V6S1 | V6S2 | V6S3 | V6S6 | |
20 nM OligoPool | 0.2 | 1.7 | 1.7 | 1.7 | 1.7 |
2X Kapa SYBR qPCR MM | 50 | 425 | 425 | 425 | 425 |
100 uM AP1V61U | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
100 uM AP2V6 | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
2 mM aa-dUTP | 3 | 25.5 | 25.5 | 25.5 | 25.5 |
H20 | 46 | 391 | 391 | 391 | 391 |
Total Volume | 100 | 850 | 850 | 850 | 850 |