Daniel:Notebook/HiResChrPaint/2013-4-22

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Revision as of 19:51, 22 April 2013 by >Djacobse
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Probe Prep S3 4/22/2013

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Purpose: Start new probe synthesis (from scratch) to better assess methods.  Getting low yields on dye coupling reaction,
but I think that the reason is I have much lower yields than Noi's original run.  Going to try the entire synthesis, focusing on only
2 sets, V4S3 and V6S3.  This way I can focus my attention better and use a more appropriately sized trial run.

Expansion qPCR

1. Set up 8 reactions for each of the 2 probes (16 reactions total)

  per rxn V4S1 V4S2 V4S3 V4S4
20 nM OligoPool 0.2 1.7 1.7 1.7 1.7
2X Kapa SYBR qPCR MM 50 425 425 425 425
100 uM AP1V41U 0.4 3.4 3.4 3.4 3.4
100 uM AP2V4 0.4 3.4 3.4 3.4 3.4
2 mM aa-dUTP 3 25.5 25.5 25.5 25.5
H20 46 391 391 391 391
Total Volume 100 850 850 850 850
  per rxn V6S1 V6S2 V6S3 V6S6
20 nM OligoPool 0.2 1.7 1.7 1.7 1.7
2X Kapa SYBR qPCR MM 50 425 425 425 425
100 uM AP1V61U 0.4 3.4 3.4 3.4 3.4
100 uM AP2V6 0.4 3.4 3.4 3.4 3.4
2 mM aa-dUTP 3 25.5 25.5 25.5 25.5
H20 46 391 391 391 391
Total Volume 100 850 850 850 850