Hosuk:LabNotes/2013-4-23
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RCA test with the product of CircLigase II reaction
Goal
- Verify that there are circularized DNA in the CircLigase II reaction sample
Experiment Procedure
- Run RCA
- S1 : CircLigase II reaction sample (18hr. @ 30°C)
- S2 : -CircLigase II sample (No CircLigase II Enzyme while CircLigase II reaction) (18hr. @ 30°C)
- Protocol
- File:RCAReationProtocolinTube.jpg
- Mixing Cy3-Adapter
- 4.5uL of each RCA sample + 0.5uL of 100uM Cy3-Adapter
- S1 : +CircLigase II + Cy3-Adapter
- S2 : -CircLigase II + Cy3-Adapter
- S3 : H2O + Cy3-Adapter