Daniel:Reagents/LambdaExonuclease

From ZhangLabWiki
Revision as of 16:36, 3 May 2013 by >Djacobse
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Lambda Exonuclease[edit]

Lambda Exonuclease datasheet (NEB): File:M0262-lambda-exonuclease.pdf

*1 unit is defined as the amount of enzyme required to produce 10 nmol of deoxyribonucleotide from double-stranded
substrate in a total reaction volume of 50 uL in 30 minutes at 37C in 1X reaction buffer with 1 ug of H3-DNA (NEB)

Heat Shock

Heat inactivate for 10 minutes at 75 C.

NEB

M0262S	1,000 units  5,000 units/ml  $63.00
M0262L	5,000 units  5,000 units/ml  $252.00