Daniel:Protocols/TBUgel

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Urea Gel Quantification

Protocol 1

1. Prerun gel for at least 20 minutes at 250 Volts
2. Prepare samples, typical setup:
  uL sample uL ladder uL TBE uL 2X urea buffer uL loaded
samples 0.5 0 5.5 6 12
ladder 1 0 0.5 5.5 6 12
ladder 2 0 1 5 6 12
3. Denature samples for 8 minutes at 65C
4. Snap cool using freezer box
5. Load samples onto gel and run for 20-25 minutes at 250V
6. Stain with 3 uL SYBR gold for at least 15 minutes