Daniel:Protocols/TBUgel
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Urea Gel Quantification
Protocol 1
1. Prerun gel for at least 20 minutes at 250 Volts 2. Prepare samples, typical setup:
uL sample | uL ladder | uL TBE | uL 2X urea buffer | uL loaded | |
samples | 0.5 | 0 | 5.5 | 6 | 12 |
ladder 1 | 0 | 0.5 | 5.5 | 6 | 12 |
ladder 2 | 0 | 1 | 5 | 6 | 12 |
3. Denature samples for 8 minutes at 65C 4. Snap cool using freezer box 5. Load samples onto gel and run for 20-25 minutes at 250V 6. Stain with 3 uL SYBR gold for at least 15 minutes