Daniel:Notebook/HiResChrPaint/2013-4-29

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Revision as of 22:12, 29 April 2013 by >Djacobse
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Probe Prep Test (Started 4/22/2013)

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TBE Gel

Purpose: As per 4/27/2013 results, I need to better understand the 
 lambda exonuclease digestion.  To this end I'm going to play around with the parameters of the reaction.  To do
 so I need several samples to work with.  I am testing/identifying samples right now that will suit my purposes.

Protocol

1. Prepared samples (1 uL sample, 5 uL TBE, 2 uL 6x dye) and loaded (7 uL) according to table
Lane 2 3 4 5 6
Sample Ladder V4S2 V6S2 V6S3 V6S3 Digest
2. Ran gel for 25 minutes at 200 V
3. Stained with 3 uL SYBR gold for 5 min

Gel Results

File:TBE 042913.jpg