Hosuk:LabNotes/2013-5-2
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Result - RT check
- based on Debugging plan (05/01)
- +RTase sample showed very strong Cy5 signal which is supposed cDNA
- comparing to -RTase result, the bright singal over the cell seemed they were cDNAs
- Most of signal came from cell structure.
- However I don't know what are the large spots. Are they also cDNAs?
- -RTase sample has very low intensity of signals in the cell, and
- not many spots were observed, which spots were outside of cells.
S2:+RTase, Brighfield, 20x | S2:+RTase, Cy5, 20x | S2:+RTase, Merged | |||
(Original) | (Original) | ||||
File:RTCheck +RTase fig08 20xobj pos04 BF exp0.2-EM00.jpg | File:RTCheck +RTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg | File:Composite +RTase fig07-08.jpg |