Kun:LabNotes/ASE/2007-10-29

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Revision as of 23:58, 29 October 2007 by KunZhang (talk | contribs) (New page: ===Exp1: Probe prep (cont.)=== *Starting from ethanol precipitated DNAs made at 10/18/2007.<br> *Pool two tubes of the same amplicons into ~100ul in total,...)
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Exp1: Probe prep (cont.)

  • Starting from ethanol precipitated DNAs made at 10/18/2007.
  • Pool two tubes of the same amplicons into ~100ul in total, add 11ul 10x Lambda Exo buffer, 4ul 5U/ul Lambda exonuclease. Incubate @ 37C for 2 hours, check 2ul of the products in both 8% TBE gels and 6% TBU gels.