Passaging hESCs on feeder
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1. Aspirate media from cells
2. Wash well once with PBS
3. Add 1ml TrpLE to each well, incubate 5 min at 37C
4. Remove TrpLE
5. Add 1ml H9 basal media and detach by scraping
6. Pipette using p1000 2-3 times to dissociate
7. Transfer 100ul (1:10) to 50ul (1:20) dissociated cells to fresh feeders (3ml total media + bFGF and Y27632)